Both microorganisms remained unstained with dark backgrounds after. Nigrosin was used to stain Micrococcus luteus and Bacillus megaterium. When an older bacteria culture undergoes nutrient depletion, its response will include production of spores. Remove the absorbent paper and decolorize it with acid-alcohol for 1 minute rinse with tap water and tap dry. In this case, endospore stain as a differential staining technique is largely used for the purposes of distinguishing between vegetative cells and endospores. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong. Endospore staining is a differential technique that selectively stains the spores and makes them distinguishable from the vegetative part of the cells. The spore is stained green and the vegetative cell is stained a pinkish red color. Endospore Stain The endospore stain Malachite green is used to dye spores produced by bacteria. An endospore stain of the cell Bacillus subtilis showing endospores as green and the vegetative cell as red Phase-bright endospores of Paenibacillus alvei imaged with phase-contrast microscopy. Saturate it with carbol-fuschin and heat fix by steaming over a boiling water bath or beaker for 5-10 minutes while adding more dye to the smear. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it.
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